When creating a PCR protocol, users normally concentrate on the template DNA, reaction mix (buffer, dNTPs, taq polymerase), primers, probes, and the thermocycler, which is the main instrument used to ...

PCR amplification can seem straightforward, but in single-cell workflows even small miscalculations can quickly complicate results and downstream analysis. Conventional PCR systems require users to ...

Programmable RNA base editors are excellent tools to modify RNA transcripts at precise locations or designated hotspots. Post editing, it is critical to provide precise and accurate quantification for ...

Real time RT-PCR can be performed using a number of commercially available kits. We currently use the QIAGEN QuantiTect Probe RT-PCR kit (#204443). The setup shown in this document is for the QIAGEN ...

This document provides detailed instructions for conducting real-time RT-PCR assays to detect the yellow fever virus, specifically the YFV 17D vaccine strain. It includes information on the ...

While the Delta variant of Covid-19 continues to swirl, many travel companies are cautiously churning back to business, albeit with modified protocols. Cruises are no exception, and Viking enlisted ...

In the summer of 1984, Henry Erlich, Glenn Horn, Randy Saiki, and myself were working on developing a DNA test for sickle cell anemia (SCA) at Cetus Corp. SCA is an autosomal dominant disease caused ...

The health, safety and medical protocols for the 2020 MLS is Back Tournament in Orlando were developed by MLS's medical department, in consultation with infectious disease specialists, government ...

Polymerase chain reaction (PCR) test kits for COVID-19 use what’s known as gene cloning – not reproductive cloning – to detect the presence or absence of the SARS-CoV-2 virus, but social media posts ...